| 质粒名称: | rAAV2-retro helper |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 81070 |
| 存储实验室: | Alla Karpova, David Schaffer |
|
载体骨架基本信息 |
|
| 载体名称: | AAV helper vector |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | AAV，未指定；辅助载体 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 30 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | rep |
| 物种来源: | adeno-associated virus |
| 插入大小: | 1865 bp |
| 基因突变: | Pro2Ala, possibly Asp613Gly |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | rAAV2-retro helper was a gift from Alla Karpova & David Schaffer (Addgene plasmid # 81070 ; http://n2t.net/addgene:81070 ; RRID:Addgene_81070) |
| 参考文献部分: | A Designer AAV Variant Permits Efficient Retrograde Access to Projection Neurons. Tervo DG, Hwang BY, Viswanathan S, Gaj T, Lavzin M, Ritola KD, Lindo S, Michael S, Kuleshova E, Ojala D, Huang CC, Gerfen CR, Schiller J, Dudman JT, Hantman AW, Looger LL, Schaffer DV, Karpova AY. Neuron. 2016 Oct 6. pii: S0896-6273(16)30580-3. doi: 10.1016/j.neuron.2016.09.021. 10.1016/j.neuron.2016.09.021 PubMed 27720486 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS rAAV2-retro helper 8369 bp ds-DNA circular SYN 26-AUG-2020
DEFINITION retrograde AAV helper vector, carrying rep and modified capsid gene.
ACCESSION .
VERSION .
KEYWORDS rAAV2-retro helper
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8369)
AUTHORS Tervo DG, Hwang BY, Viswanathan S, Gaj T, Lavzin M, Ritola KD, Lindo
S, Michael S, Kuleshova E, Ojala D, Huang CC, Gerfen CR, Schiller J,
Dudman JT, Hantman AW, Looger LL, Schaffer DV, Karpova AY
TITLE A Designer AAV Variant Permits Efficient Retrograde Access to
Projection Neurons.
JOURNAL Neuron. 2016 Oct 6. pii: S0896-6273(16)30580-3. doi:
10.1016/j.neuron.2016.09.021.
PUBMED 27720486
REFERENCE 2 (bases 1 to 8369)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 26, 2020 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8369
/organism="synthetic DNA construct"
/mol_type="other DNA"
repeat_region 44..146
/label=ITR
/note="inverted terminal repeat of human adenovirus
serotype 5"
rep_origin 5186..5699
/direction=RIGHT
/label=M13 ori
/note="M13 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5273..5292)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 5483..5504
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(6181..6200)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 6300..6322
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(6360..6378)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 6446..6550
/gene="bla"
/label=AmpR promoter
CDS 6551..7411
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(6769..6788)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 7582..8170
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 8071..8090
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 8324..8341
/label=L4440
/note="L4440 vector, forward primer"
ORIGIN
1 cgcctctccc cgcgcgttgg ccgattcatt aatgcagaat tcccatcatc aataatatac
61 cttattttgg attgaagcca atatgataat gagggggtgg agtttgtgac gtggcgcggg
121 gcgtgggaac ggggcgggtg acgtagtagc tctagaggtc ctgtattaga ggtcacgtga
181 gtgttttgcg acattttgcg acaccatgtg gtcacgctgg gtatttaagc ccgagtgagc
241 acgcagggtc tccattttga agcgggaggt ttgaacgcgc agccaccacg gcggggtttt
301 acgagattgt gattaaggtc cccagcgacc ttgacgagca tctgcccggc atttctgaca
361 gctttgtgaa ctgggtggcc gagaaggaat gggagttgcc gccagattct gacatggatc
421 tgaatctgat tgagcaggca cccctgaccg tggccgagaa gctgcagcgc gactttctga
481 cggaatggcg ccgtgtgagt aaggccccgg aggccctttt ctttgtgcaa tttgagaagg
541 gagagagcta cttccacatg cacgtgctcg tggaaaccac cggggtgaaa tccatggttt
601 tgggacgttt cctgagtcag attcgcgaaa aactgattca gagaatttac cgcgggatcg
661 agccgacttt gccaaactgg ttcgcggtca caaagaccag aaatggcgcc ggaggcggga
721 acaaggtggt ggatgagtgc tacatcccca attacttgct ccccaaaacc cagcctgagc
781 tccagtgggc gtggactaat atggaacagt atttaagcgc ctgtttgaat ctcacggagc
841 gtaaacggtt ggtggcgcag catctgacgc acgtgtcgca gacgcaggag cagaacaaag
901 agaatcagaa tcccaattct gatgcgccgg tgatcagatc aaaaacttca gccaggtaca
961 tggagctggt cgggtggctc gtggacaagg ggattacctc ggagaagcag tggatccagg
1021 aggaccaggc ctcatacatc tccttcaatg cggcctccaa ctcgcggtcc caaatcaagg
1081 ctgccttgga caatgcggga aagattatga gcctgactaa aaccgccccc gactacctgg
1141 tgggccagca gcccgtggag gacatttcca gcaatcggat ttataaaatt ttggaactaa
1201 acgggtacga tccccaatat gcggcttccg tctttctggg atgggccacg aaaaagttcg
1261 gcaagaggaa caccatctgg ctgtttgggc ctgcaactac cgggaagacc aacatcgcgg
1321 aggccatagc ccacactgtg cccttctacg ggtgcgtaaa ctggaccaat gagaactttc
1381 ccttcaacga ctgtgtcgac aagatggtga tctggtggga ggaggggaag atgaccgcca
1441 aggtcgtgga gtcggccaaa gccattctcg gaggaagcaa ggtgcgcgtg gaccagaaat
1501 gcaagtcctc ggcccagata gacccgactc ccgtgatcgt cacctccaac accaacatgt
1561 gcgccgtgat tgacgggaac tcaacgacct tcgaacacca gcagccgttg caagaccgga
1621 tgttcaaatt tgaactcacc cgccgtctgg atcatgactt tgggaaggtc accaagcagg
1681 aagtcaaaga ctttttccgg tgggcaaagg atcacgtggt tgaggtggag catgaattct
1741 acgtcaaaaa gggtggagcc aagaaaagac ccgcccccag tgacgcagat ataagtgagc
1801 ccaaacgggt gcgcgagtca gttgcgcagc catcgacgtc agacgcggaa gcttcgatca
1861 actacgcaga caggtaccaa aacaaatgtt ctcgtcacgt gggcatgaat ctgatgctgt
1921 ttccctgcag acaatgcgag agaatgaacc agaattcaaa tatctgcttc actcacggac
1981 agaaagactg tttagagtgc tttcccgtgt cagaatctca acccgtttct gtcgtcaaaa
2041 aggcgtatca gaaactgtgc tacattcatc atatcatggg aaaggtgcca gacgcttgca
2101 ctgcctgcga tctggtcaat gtgggtttgg atgactgcat ctttgaacaa taaatgattt
2161 aaatcaggta tggctgccga tggttatctt ccagattggc tcgaggacac tctctctgaa
2221 ggaataagac agtggtggaa gctcaaacct ggcccaccac caccaaagcc cgcagagcgg
2281 cataaggacg acagcagggg tcttgtgctt cctgggtaca agtacctcgg acccttcaac
2341 ggactcgaca agggagagcc ggtcaacgag gcagacgccg cggccctcga gcacgacaaa
2401 gcctacgacc ggcagctcga cagcggagac aacccgtacc tcaagtacaa ccacgccgac
2461 gcggagtttc aggagcgcct taaagaagat acgtcttttg ggggcaacct cggacgagca
2521 gtcttccagg cgaaaaagag ggttcttgaa cctctgggcc tggttgagga acctgttaag
2581 acggctccgg gaaaaaagag gccggtagag cactctcctg tggagccaga ctcctcctcg
2641 ggaaccggaa aggcgggcca gcagcctgca agaaaaagat tgaattttgg tcagactgga
2701 gacgcagact cagtacctga cccccagcct ctcggacagc caccagcagc cccctctggt
2761 ctgggaacta atacgatggc tacaggcagt ggcgcaccaa tggcagacaa taacgagggc
2821 gccgacggag tgggtaattc ctcgggaaat tggcattgcg attccacatg gatgggcgac
2881 agagtcatca ccaccagcac ccgaacctgg gccctgccca cctacaacaa ccacctctac
2941 aaacaaattt ccagccaatc aggagcctcg aacgacaatc actactttgg ctacagcacc
3001 ccttgggggt attttgactt caacagattc cactgccact tttcaccacg tgactggcaa
3061 agactcatca acaacaactg gggattccga cccaagagac tcaacttcaa gctctttaac
3121 attcaagtca aagaggtcac gcagaatgac ggtacgacga cgattgccaa taaccttacc
3181 agcacggttc aggtgtttac tgactcggag taccagctcc cgtacgtcct cggctcggcg
3241 catcaaggat gcctcccgcc gttcccagca gacgtcttca tggtgccaca gtatggatac
3301 ctcaccctga acgacgggag tcaggcagta ggacgctctt cattttactg cctggagtac
3361 tttccttctc agatgctgcg taccggaaac aactttacct tcagctacac ttttgaggac
3421 gttcctttcc acagcagcta cgctcacagc cagagtctgg accgtctcat gaatcctctc
3481 atcgaccagt acctgtatta cttgagcaga acaaacactc caagtggaac caccacgcag
3541 tcaaggcttc agttttctca ggccggagcg agtgacattc gggaccagtc taggaactgg
3601 cttcctggac cctgttaccg ccagcagcga gtatcaaaga catctgcgga taacaacaac
3661 agtgaatact cgtggactgg agctaccaag taccacctca atggcagaga ctctctggtg
3721 aatccgggcc cggccatggc aagccacaag gacgatgaag aaaagttttt tcctcagagc
3781 ggggttctca tctttgggaa gcaaggctca gagaaaacaa atgtggacat tgaaaaggtc
3841 atgattacag acgaagagga aatcaggaca accaatcccg tggctacgga gcagtatggt
3901 tctgtatcta ccaacctcca gagaggcaac ctagcagacc aagactacac aaaaactgct
3961 aggcaagcag ctaccgcaga tgtcaacaca caaggcgttc ttccaggcat ggtctggcag
4021 gacagagatg tgtaccttca ggggcccatc tgggcaaaga ttccacacac ggacggacat
4081 tttcacccct ctcccctcat gggtggattc ggacttaaac accctcctcc ccagattctc
4141 atcaagaaca ccccggtacc tgcgaatcct tcgaccacct tcagtgcggc aaagtttgct
4201 tccttcatca cacagtactc cacgggacag gtcagcgtgg agatcgagtg ggagctgcag
4261 aaggaaaaca gcaaacgctg gaatcccgaa attcagtaca cttccaacta caacaagtct
4321 attaatgtgg actttactgt ggacactaat ggcgtgtatt cagagcctcg ccccattggc
4381 accagatacc tgactcgtaa tctgtaattg cggccgcttg ttaatcaata aaccgtttaa
4441 ttcgtttcag ttgaactttg gtctctgcgt atttctttct tatctagttt ccatgctcta
4501 gaggtcctgt attagaggtc acgtgagtgt tttgcgacat tttgcgacac catgtggtca
4561 cgctgggtat ttaagcccga gtgagcacgc agggtctcca ttttgaagcg ggaggtttga
4621 acgcgcagcc accacggcgg ggttttacga gattgtgatt aaggtcccca gcgaccttga
4681 cgagcatctg cccggcattt ctgacagctt tgtgaactgg gtggccgaga aggaatggga
4741 gttgccgcca gattctgaca tggatctgaa tctgattgag caggcacccc tgaccgtggc
4801 cgagaagctg catcgctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
4861 gttgcgcagc ctgaatggcg aatggcgatt ccgttgcaat ggctggcggt aatattgttc
4921 tggatattac cagcaaggcc gatagtttga gttcttctac tcaggcaagt gatgttatta
4981 ctaatcaaag aagtattgcg acaacggtta atttgcgtga tggacagact cttttactcg
5041 gtggcctcac tgattataaa aacacttctc aggattctgg cgtaccgttc ctgtctaaaa
5101 tccctttaat cggcctcctg tttagctccc gctctgattc taacgaggaa agcacgttat
5161 acgtgctcgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt
5221 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
5281 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
5341 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
5401 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
5461 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
5521 atctcggtct attcttttga tttataaggg attttgccga tttcggcata ttggttaaaa
5581 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
5641 taaatatttg cttatacaat cttcctgttt ttggggcttt tctgattatc aaccggggta
5701 catatgattg acatgctagt tttacgatta ccgttcatcg attctcttgt ttgctccaga
5761 ctctcaggca atgacctgat agcctttgta gagacctctc aaaaatagct accctctccg
5821 gcatgaattt atcagctaga acggttgaat atcatattga tggtgatttg actgtctccg
5881 gcctttctca cccgtttgaa tctttaccta cacattactc aggcattgca tttaaaatat
5941 atgagggttc taaaaatttt tatccttgcg ttgaaataaa ggcttctccc gcaaaagtat
6001 tacagggtca taatgttttt ggtacaaccg atttagcttt atgctctgag gctttattgc
6061 ttaattttgc taattctttg ccttgcctgt atgatttatt ggatgttgga atcgcctgat
6121 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
6181 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
6241 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
6301 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
6361 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
6421 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
6481 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
6541 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
6601 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
6661 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
6721 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
6781 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
6841 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
6901 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
6961 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
7021 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
7081 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
7141 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
7201 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
7261 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
7321 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
7381 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
7441 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
7501 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
7561 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
7621 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
7681 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
7741 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
7801 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
7861 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
7921 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
7981 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
8041 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
8101 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
8161 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
8221 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
8281 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
8341 ggaagcggaa gagcgcccaa tacgcaaac
//
